Instruments and software the bioplex pro assays described in this manual are compatible with all currently available luminex based life science research instruments. Bioplex manager software can be used for data analysis. When performing routine maintenance, turn power to the luminex 200 analyzer off and disconnect the power cord. Luminex technology employs microsphere beads, either polystyrene or. The procedures are presented as stepbystep instructions and assume basic knowledge of xponent software and xmap technology. Luminex xmap technology life science research milliporesigma.
Luminex 100200 sets the standard for multiplexing, with over 11,000 units sold globally and more than fifty 510kcleared assays. Oct 12, 2012 magpix system how to analyze protein data thermo fisher scientific. The software should be fully licensed or uninstalled before the trial period expires. Magpix multiplexing instrument from luminex corporation. The ability to perform up to 100 different tests in a single reaction volume and use either magnetic beads or plain polystyrene beads makes this instrument a popular choice for laboratories needing greater flexibility. Customercentric development and extensive field testing with customers has resulted in a user experience which is a significant step forward in the market place. Repair modified or deleted xponent system files by uninstalling and reinstalling the xponent software. We also operate and maintain two luminex flexmap 3d systems with xponent software in our boston, ma laboratory, and both are 21 cfr part 11 compliant. This information is presented to support researchers who use magpix, 100200, and flexmap 3d luminex systems. See the xponent license agreement for more information. The protocol applied in all runs was performed at 58c with a minimum of 50 beads counted for each bead region. Follow the instructions in the ivd kits product insert and use the software protocol provided.
Magpix features selfcleaning routines and magnetic bead compatibility with. Multiplexing on xmap beads is a great approach for looking at several analytes. Multiplex detection of bacteria associated with normal. Adjust probe height if probe has been removed page 115 of xponent software manual a. Luminex xmap technology, the basis for our milliplex map assay kits, is a powerful platform for multiplex detection of proteins in a single biological sample. Overview the magpix immunoassay system employs leds to excite fluoresced beads in up to 50 wells and detects. The software s graphical user interface follows the typical assay workflow, but can be customized to meet the needs of a specific laboratory. How luminex xmap technology works sets of magnetic microspheres are colorcoded with combinations of red and infrared fluorophores. Designed for easeofuse, the software allows either a novice or advanced user to set up and run an assay in a minimal amount of time. Fast read time, compatibility with 96 and 384well plates, and ease of integration with frontend automation systems make the flexmap 3d the platform of choice for highthroughput applications. Here we compare the results obtained with the luminex magpix. Luminexs xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Bring your biomarkers to life with the established leaders.
Serological markers are potentially useful tools for monitoring the progress of malaria control programs, but a better understanding of antibody response dynamics is necessary. Samples were then read on either a luminex magpix or a lx100 reader with xponent software using the parameters outlined in the assay kit instructions. Feb 03, 2017 luminex xponent software provides data acquisition and analysis for luminex xmap instruments. This protocol is a stepbystep method for setting up the xponent 3. Designed for easeofuse, xponent enables both new and advanced users to set up and run assays in a minimal amount of time. Browse luminex accessories and learn about our luminex software, luminex beads compactivity and more.
Luminex xmap technology is based on polystyrene or paramagnetic microspheres, or beads, that are internally dyed with red and infrared fluorophores of differing intensities. This compact multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96wellplate in just 60 minutes. A beads only control extra sample, was included each time the assay was run for background signal analysis. Magpix magpix is the simplest, most affordable, and most compact of luminexs xmap instruments. Optional software modules all optional modules can be added when purchasing a system. Luminex 200 instrument, xy platform, sheath fluid delivery system, xponent 3. Feature introduction to luminex xmap technology and.
Luminex lx200 with xponent software zeus scientific. All microspheres are general use components for further manufacture of xmap bioassays. Intended use perform multiple assays with a single sample with the athena multilyte test analyzer, built by luminex corporation on the luminex 100200 system using xmap technology. A minimum of 35 beads per species were counted and analysed per sample. Combined species identification, genotyping, and drug. Like the luminex 200 and flexmap 3d systems, the magpix system uses xponent software, offering performance and user interaction similar to these more expensive flow cytometrybased systems. Clinical diagnostics instruments magpix multiplexing luminex. Do not dilute magpix calibration or verification beads, or the fluidics 1 and fluidics 2 beads.
Each dyed bead is given a unique number, known as a bead region, allowing the differentiation of beads. How luminex xmap technology works the luminex system is the combination of four core technologies. Luminex technology employs microsphere beads, either polystyrene or paramagnetic, that are color. These software update are only necessary for magpix systems running xponent software version 4. The ability to perform up to 100 different tests in a single reaction volume and use either magnetic beads or plain polysty. The elx50 microplate strip washer biotek instruments used in these experiments was configured with a 96well flat magnet pn 7103016. Im magpix system werden magnetische beads magplex verwendet, welche eine. Luminex multiplexing instruments life science research. Bioplex 3d suspension array system life science research. Its selfcleaning routines and magnetic bead compatibility with magplex beads make magpix extremely easy to learn and use. Magpix is the simplest, most affordable, and most compact of luminexs xmap instruments.
This flexible software package controls the, luminex 200, flexmap 3d, and magpix. The results were expressed as arbitrary units of median fluorescence intensity mfi. Prn luminex corporation launches xponenttm software. Magpix multiplexing instrument from luminex corporation description the magpix detects up to 50 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Finally, all analyses were performed on a magpix device luminex corporation, austin, usa equipped with the xponent for magpix v4. Enduser license agreement eula for luminex xponent software this luminex enduser license agreement eula is a legal agreement between you either an individual or a single entity, also referred herein as you the enduser and luminex corporation luminex regarding the use of the xponent software product provided to. A number of labs in the far east, europe and north america have already validated this panel and are planning to go live with it this week. These assays are compatible with both vacuum and magnetic wash stations, as well as other luminex xmap platforms. Luminex provides update on sarscov2 validation testing. Automated washing of multiplex bead based assays for the. This multiplex, fluorescent, beadbased system offers a simple and efficient alternative to ifa and elisa testing. Can be read on a luminex machine with xponent software, flexmap3d. Automated washing of multiplex bead based assays for the luminex magpix reader system comparison of luminex 100 and magpix luminex readers a p p l i c a t i o n n o t e the magpix reader system has been developed exclusively for use with magnetic magplex microspheres. Please contact luminex technical support to obtain a permanent license.
Luminex magpix is the simplest, most affordable, and most portable. The luminex xmap technology offers significant advantages over traditional bioassays, such as the elisa, by offering highthroughput. Luminex s xponent software was developed with experts in human factors and ergonomic design. The xponent application is licensed in trial mode for 30 days. All luminex instruments come with xponent data analysis software that can be used.
Luminex technology employs microsphere beads, either polystyrene or paramagnetic, that are colorcoded. Median fluorescence intensity mfi signals from the samples were measured on the magpix equipment using the xponent 5. Click the desired analytes bead id in the numbered analyte grid. Luminex 200 system user manual the following label appears on the back of the luminex 200 instrument. The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. The softwares graphical user interface follows the typical assay workflow, but can be customized to meet the needs of a specific laboratory. Luminex luminex xmap technology highly flexible, costeffective, and efficient as an alternative to traditional bioassays, abl offers the luminex 200 system assay platform, a flexible, beadbased detection system incorporating the principles of flow cytometry. This system contains electrical and mechanical components that, if handled improperly, are potentially harmful. Luminex recommends that you contact luminex technical support before uninstalling and reinstalling xponent. Software updates are available for your luminex magpix system. What software version is compatible with bioplex 3d system. Luminex 200 system with xponent software from thermo. The third component is the xponent software, which is designed for. Bacterial vaginosis bv is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease.
After the purchase of the system, all optional upgrades can be purchased directly through luminex corporation. All luminex instrumentation sold by biotechne arrives pre installed with xponent data. Designed for easeofuse, the software allows the novice or occasional user and advanced user alike, to set up and run an assay in a minimal amount of time. The samples and standards were then resuspended in 100. Luminex multiplexing technology consists of bead based immunoassays using xmap beads, high plex multiplexing instrument, and powerful software. These beads are universally compatible with all xmap instruments. The systems xponent software is designed for protocolbased data acquisition with robust data regression analysis. The millipore software and bioplex manager can use xponent output. Luminex luminex xmap technology highly flexible, costeffective, and efficient as an alternative to traditional bioassays, abl offers the luminex 200 system assay platform, a flexible, bead based detection system incorporating the principles of flow cytometry.
Systems include the luminex magpix, luminex 200, and luminex flexmap 3d. Lmnx introduces two new key products xponent, a new, more intuitive software interface which simplifies. The use of a magnetic beadbased immunoassay mba is advantageous compared to elisa, due to its multiplexing capacity, but limited information is available on the standardization and validation of this assay. Luminex multiplexing instruments offer the only solution for applications requiring both high density and high throughput at the same time.
Turn on instrument there are two power switches, one on the back and one on the front. State of the art optics, electronics, and software make the labscan systems capable of rapidly processing up to 96 samples. Luminex 200 system with xponent software from thermo fisher scientific description the luminex 200 system is a compact analyzer that performs up to 100 assays simultaneously in a single well of a microtiter plate. Bring your biomarkers to life with the established leaders in. The luminex 100200 analyzer integrates key xmap detection components such as lasers, optics, fluidics and highspeed digital signal processors. Fast read time, compatibility with 96 and 384well plates, and ease of integration with frontend automation systems make the flexmap 3d the. Pdf evaluation of fluorescence microsphere immunoassay. The labscan3d and labscan 100 systems are based on luminex xmap technology and use luminex xponent software for data acquisition. Featuring a flexible, openarchitecture design, xmap technology can be configured to perform a wide variety of protein or nuclei. The luminex 100200 system is a flexible analyzer based on the principles of flow cytometry.
Magplex microspheres open the door to luminex s magpix instrument, which was designed as a simple and easy to use instrument with a smaller foot print. The ability to use either magnetic or polystyrene beads makes this instrument a popular choice for laboratories needing greater flexibility. Antibody responses were expressed in median fluorescence intensity mfi per sample as stated by manufacturers instructions. Technologies xmap microspheres, the flow cytometrybased luminex analyzer, and the xponent software luminex can provide clients with a heat map style analysis across a broad panel of analytes. The new magpix multiplexing system thermo fisher scientific us. Used luminex magpix multiplex reader immunology for sale.
The luminex 100200 instruments, operating on xponent software, are. Assays can be read and analyzed with either bioplex manager software or luminex xponent software see read plate in section 7. The ability to use either magnetic or polystyrene beads makes this instrument a. Magnetic bead magplex applications, lis connectivity via astm protocol. Watch video on luminex xmap technology as a luminex partner, we are a preferred distributor of the entire luminex portfolio of multiplexing instruments, including luminex 200, flexmap 3d and the. Introduction to xmap technology and milliplex assay panels. If the trial period expires, the application will become inoperable.
Under no circumstances should you remove the luminex 200 analyzer cover. The new luminex magpix system is an affordable, compact fluorescent. Luminex assays supportgetting started thermo fisher. In contrast to the lactobacillus dominated normal vaginal microbiota, bv is characterized by a lack of lactobacilli and an abundance of. Each bead set is coated with a reagent specific to a particular bioassay, allowing the capture and detection of several specific analytes from a single sample. Development and performance assessment of a luminex xmap. Luminex 200 system with xponent software from thermo fisher. We have multiple data analysis software options, and pride ourselves on being versatile with data analysis. On the pc desktop, click the luminex xponent icon, or click start all programs. Luminex xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Following the final wash, each well of beads were resuspended in 125. A and b the luminex magpix system has up to 50 spectrally distinct bead sets to allow for the simultaneous. Ensure that bead counts are at least 50 per analyte. Luminex 100200 for life science research standard for.
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